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mct4 philp lab  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mct4 philp lab
    List of primers used for qPCR and genotyping
    Mct4 Philp Lab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mct4+philp+lab/pmc07849204-139-57-65?v=Cell+Signaling+Technology+Inc
    Average 92 stars, based on 29 article reviews
    mct4 philp lab - by Bioz Stars, 2026-08
    92/100 stars

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    1) Product Images from "Role of monocarboxylate transporters in regulating metabolic homeostasis in the outer retina: Insight gained from cell-specific Bsg deletion"

    Article Title: Role of monocarboxylate transporters in regulating metabolic homeostasis in the outer retina: Insight gained from cell-specific Bsg deletion

    Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

    doi: 10.1096/fj.201902961R

    List of primers used for qPCR and genotyping
    Figure Legend Snippet: List of primers used for qPCR and genotyping

    Techniques Used:

    List of antibodies used in this study and dilution
    Figure Legend Snippet: List of antibodies used in this study and dilution

    Techniques Used:

    rBSG1 is required MCT1 and MCT4 in rod photoreceptor cells. A, qPCR showing reduced levels of Bsg1 in RodΔBsg retina without compensatory increases in levels of other Ig superfamily members. Bars indicate average ± SEM for N = 4 mice. B, Immunofluorescence of BSG (red) and MCT1 (green) of frozen sections of eyes from control and RodΔBsg mice. Loss of BSG1 resulted in loss of MCT1 in inner segments. MCT1 was still detected in rods (arrows) but was not trafficked to the plasma membrane (Scale bar = 25 µm). Asterisks indicate cones which retain both BSG1 and MCT1. Data are representative of N = 3 experiments. C, Western blots of detergent soluble lysates from RodΔBsg retinas confirms genetic deletion of Bsg from rod photoreceptors targets MCTs for degradation. Blots are representative of N = 8 mice. D, Western blot quantification of MCT1 and MCT4 compared to β-tubulin of control and RodΔBsg samples. E, Comparison of Log2(CPM) values of Slc16a1 (MCT1) and Slc16a3 (MCT4) between rods and cones at P28 from GSE74660. F, Immunoprecipitation of MCT1 and MCT4 shows enrichment of rBSG1 in control as compared to RodΔBsg retina. Data are representative of N = 3 experiments
    Figure Legend Snippet: rBSG1 is required MCT1 and MCT4 in rod photoreceptor cells. A, qPCR showing reduced levels of Bsg1 in RodΔBsg retina without compensatory increases in levels of other Ig superfamily members. Bars indicate average ± SEM for N = 4 mice. B, Immunofluorescence of BSG (red) and MCT1 (green) of frozen sections of eyes from control and RodΔBsg mice. Loss of BSG1 resulted in loss of MCT1 in inner segments. MCT1 was still detected in rods (arrows) but was not trafficked to the plasma membrane (Scale bar = 25 µm). Asterisks indicate cones which retain both BSG1 and MCT1. Data are representative of N = 3 experiments. C, Western blots of detergent soluble lysates from RodΔBsg retinas confirms genetic deletion of Bsg from rod photoreceptors targets MCTs for degradation. Blots are representative of N = 8 mice. D, Western blot quantification of MCT1 and MCT4 compared to β-tubulin of control and RodΔBsg samples. E, Comparison of Log2(CPM) values of Slc16a1 (MCT1) and Slc16a3 (MCT4) between rods and cones at P28 from GSE74660. F, Immunoprecipitation of MCT1 and MCT4 shows enrichment of rBSG1 in control as compared to RodΔBsg retina. Data are representative of N = 3 experiments

    Techniques Used: Immunofluorescence, Control, Clinical Proteomics, Membrane, Western Blot, Comparison, Immunoprecipitation

    Retinal phenotype of the MCT4−/− mouse A) Western blot analysis of control, MCT4−/−, and RodΔBsg retinas. Blots are representative of N = 3 mice. B) Lactate efflux from control (N = 6), MCT4−/− (N = 3), and RodΔBsg retinas (N = 3). Luminance-response functions for the major components of the C) dark-adapted and D) light-adapted ERGs obtained from control and MCT4−/− mice. Data points indicate average (±SEM) for 12 mice
    Figure Legend Snippet: Retinal phenotype of the MCT4−/− mouse A) Western blot analysis of control, MCT4−/−, and RodΔBsg retinas. Blots are representative of N = 3 mice. B) Lactate efflux from control (N = 6), MCT4−/− (N = 3), and RodΔBsg retinas (N = 3). Luminance-response functions for the major components of the C) dark-adapted and D) light-adapted ERGs obtained from control and MCT4−/− mice. Data points indicate average (±SEM) for 12 mice

    Techniques Used: Western Blot, Control



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    mct4 philp lab  (Cell Signaling Technology Inc)
    92
    Cell Signaling Technology Inc mct4 philp lab
    List of primers used for qPCR and genotyping
    Mct4 Philp Lab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mct4+philp+lab/pmc07849204-139-57-65?v=Cell+Signaling+Technology+Inc
    Average 92 stars, based on 1 article reviews
    mct4 philp lab - by Bioz Stars, 2026-08
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    List of primers used for qPCR and genotyping

    Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

    Article Title: Role of monocarboxylate transporters in regulating metabolic homeostasis in the outer retina: Insight gained from cell-specific Bsg deletion

    doi: 10.1096/fj.201902961R

    Figure Lengend Snippet: List of primers used for qPCR and genotyping

    Article Snippet: Slides were imaged on an LSM 780 NLO laser scanning microscope (Carl Zeiss, Oberkochen, Germany) using ApoPlan ×63/1.4 objective and EC NeoPlan ×10/0.3 objective. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Antibody Company Catalog WB dilution IF dilution BSG(CD147) Santa Cruz Sc-9757 1:5000 1:500 MCT1 Philp Lab (7–12) 1:2000 1:250 MCT3 Philp Lab (7–12) 1:5000 – MCT4 Philp Lab (7–12) 1:2000 – β-actin Cell Signaling 4967 1:2000 β-tubulin Millipore Sigma T4026 1:5000 – Cre Millipore MAB-3120 – 1:100 Cone Arrestin Millipore AB15282 – 1:100 ZO-1 Zymed Laboratories Inc. 61-7300 – 1:100 Bovine anti-goat IgG-HRP Jackson Immuno Research 115-475-207 1:2000 – Goat anti-rabbit IgG-HRP Jackson Immuno Research 111-035-144 1:2000 – Alexa Fluor Donkey anti Rabbit 488 ThermoFisher Scientific A21206 – 1:500 Alexa Fluor Donkey anti Goat 555 ThermoFisher Scientific A21432 – 1:500 Alexa Fluor Donkey anti Mouse 546 ThermoFisher Scientific A10036 – 1:500 Open in a separate window List of antibodies used in this study and dilution 2.6 |.

    Techniques:

    List of antibodies used in this study and dilution

    Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

    Article Title: Role of monocarboxylate transporters in regulating metabolic homeostasis in the outer retina: Insight gained from cell-specific Bsg deletion

    doi: 10.1096/fj.201902961R

    Figure Lengend Snippet: List of antibodies used in this study and dilution

    Article Snippet: Slides were imaged on an LSM 780 NLO laser scanning microscope (Carl Zeiss, Oberkochen, Germany) using ApoPlan ×63/1.4 objective and EC NeoPlan ×10/0.3 objective. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Antibody Company Catalog WB dilution IF dilution BSG(CD147) Santa Cruz Sc-9757 1:5000 1:500 MCT1 Philp Lab (7–12) 1:2000 1:250 MCT3 Philp Lab (7–12) 1:5000 – MCT4 Philp Lab (7–12) 1:2000 – β-actin Cell Signaling 4967 1:2000 β-tubulin Millipore Sigma T4026 1:5000 – Cre Millipore MAB-3120 – 1:100 Cone Arrestin Millipore AB15282 – 1:100 ZO-1 Zymed Laboratories Inc. 61-7300 – 1:100 Bovine anti-goat IgG-HRP Jackson Immuno Research 115-475-207 1:2000 – Goat anti-rabbit IgG-HRP Jackson Immuno Research 111-035-144 1:2000 – Alexa Fluor Donkey anti Rabbit 488 ThermoFisher Scientific A21206 – 1:500 Alexa Fluor Donkey anti Goat 555 ThermoFisher Scientific A21432 – 1:500 Alexa Fluor Donkey anti Mouse 546 ThermoFisher Scientific A10036 – 1:500 Open in a separate window List of antibodies used in this study and dilution 2.6 |.

    Techniques:

    rBSG1 is required MCT1 and MCT4 in rod photoreceptor cells. A, qPCR showing reduced levels of Bsg1 in RodΔBsg retina without compensatory increases in levels of other Ig superfamily members. Bars indicate average ± SEM for N = 4 mice. B, Immunofluorescence of BSG (red) and MCT1 (green) of frozen sections of eyes from control and RodΔBsg mice. Loss of BSG1 resulted in loss of MCT1 in inner segments. MCT1 was still detected in rods (arrows) but was not trafficked to the plasma membrane (Scale bar = 25 µm). Asterisks indicate cones which retain both BSG1 and MCT1. Data are representative of N = 3 experiments. C, Western blots of detergent soluble lysates from RodΔBsg retinas confirms genetic deletion of Bsg from rod photoreceptors targets MCTs for degradation. Blots are representative of N = 8 mice. D, Western blot quantification of MCT1 and MCT4 compared to β-tubulin of control and RodΔBsg samples. E, Comparison of Log2(CPM) values of Slc16a1 (MCT1) and Slc16a3 (MCT4) between rods and cones at P28 from GSE74660. F, Immunoprecipitation of MCT1 and MCT4 shows enrichment of rBSG1 in control as compared to RodΔBsg retina. Data are representative of N = 3 experiments

    Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

    Article Title: Role of monocarboxylate transporters in regulating metabolic homeostasis in the outer retina: Insight gained from cell-specific Bsg deletion

    doi: 10.1096/fj.201902961R

    Figure Lengend Snippet: rBSG1 is required MCT1 and MCT4 in rod photoreceptor cells. A, qPCR showing reduced levels of Bsg1 in RodΔBsg retina without compensatory increases in levels of other Ig superfamily members. Bars indicate average ± SEM for N = 4 mice. B, Immunofluorescence of BSG (red) and MCT1 (green) of frozen sections of eyes from control and RodΔBsg mice. Loss of BSG1 resulted in loss of MCT1 in inner segments. MCT1 was still detected in rods (arrows) but was not trafficked to the plasma membrane (Scale bar = 25 µm). Asterisks indicate cones which retain both BSG1 and MCT1. Data are representative of N = 3 experiments. C, Western blots of detergent soluble lysates from RodΔBsg retinas confirms genetic deletion of Bsg from rod photoreceptors targets MCTs for degradation. Blots are representative of N = 8 mice. D, Western blot quantification of MCT1 and MCT4 compared to β-tubulin of control and RodΔBsg samples. E, Comparison of Log2(CPM) values of Slc16a1 (MCT1) and Slc16a3 (MCT4) between rods and cones at P28 from GSE74660. F, Immunoprecipitation of MCT1 and MCT4 shows enrichment of rBSG1 in control as compared to RodΔBsg retina. Data are representative of N = 3 experiments

    Article Snippet: Slides were imaged on an LSM 780 NLO laser scanning microscope (Carl Zeiss, Oberkochen, Germany) using ApoPlan ×63/1.4 objective and EC NeoPlan ×10/0.3 objective. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Antibody Company Catalog WB dilution IF dilution BSG(CD147) Santa Cruz Sc-9757 1:5000 1:500 MCT1 Philp Lab (7–12) 1:2000 1:250 MCT3 Philp Lab (7–12) 1:5000 – MCT4 Philp Lab (7–12) 1:2000 – β-actin Cell Signaling 4967 1:2000 β-tubulin Millipore Sigma T4026 1:5000 – Cre Millipore MAB-3120 – 1:100 Cone Arrestin Millipore AB15282 – 1:100 ZO-1 Zymed Laboratories Inc. 61-7300 – 1:100 Bovine anti-goat IgG-HRP Jackson Immuno Research 115-475-207 1:2000 – Goat anti-rabbit IgG-HRP Jackson Immuno Research 111-035-144 1:2000 – Alexa Fluor Donkey anti Rabbit 488 ThermoFisher Scientific A21206 – 1:500 Alexa Fluor Donkey anti Goat 555 ThermoFisher Scientific A21432 – 1:500 Alexa Fluor Donkey anti Mouse 546 ThermoFisher Scientific A10036 – 1:500 Open in a separate window List of antibodies used in this study and dilution 2.6 |.

    Techniques: Immunofluorescence, Control, Clinical Proteomics, Membrane, Western Blot, Comparison, Immunoprecipitation

    Retinal phenotype of the MCT4−/− mouse A) Western blot analysis of control, MCT4−/−, and RodΔBsg retinas. Blots are representative of N = 3 mice. B) Lactate efflux from control (N = 6), MCT4−/− (N = 3), and RodΔBsg retinas (N = 3). Luminance-response functions for the major components of the C) dark-adapted and D) light-adapted ERGs obtained from control and MCT4−/− mice. Data points indicate average (±SEM) for 12 mice

    Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

    Article Title: Role of monocarboxylate transporters in regulating metabolic homeostasis in the outer retina: Insight gained from cell-specific Bsg deletion

    doi: 10.1096/fj.201902961R

    Figure Lengend Snippet: Retinal phenotype of the MCT4−/− mouse A) Western blot analysis of control, MCT4−/−, and RodΔBsg retinas. Blots are representative of N = 3 mice. B) Lactate efflux from control (N = 6), MCT4−/− (N = 3), and RodΔBsg retinas (N = 3). Luminance-response functions for the major components of the C) dark-adapted and D) light-adapted ERGs obtained from control and MCT4−/− mice. Data points indicate average (±SEM) for 12 mice

    Article Snippet: Slides were imaged on an LSM 780 NLO laser scanning microscope (Carl Zeiss, Oberkochen, Germany) using ApoPlan ×63/1.4 objective and EC NeoPlan ×10/0.3 objective. table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Antibody Company Catalog WB dilution IF dilution BSG(CD147) Santa Cruz Sc-9757 1:5000 1:500 MCT1 Philp Lab (7–12) 1:2000 1:250 MCT3 Philp Lab (7–12) 1:5000 – MCT4 Philp Lab (7–12) 1:2000 – β-actin Cell Signaling 4967 1:2000 β-tubulin Millipore Sigma T4026 1:5000 – Cre Millipore MAB-3120 – 1:100 Cone Arrestin Millipore AB15282 – 1:100 ZO-1 Zymed Laboratories Inc. 61-7300 – 1:100 Bovine anti-goat IgG-HRP Jackson Immuno Research 115-475-207 1:2000 – Goat anti-rabbit IgG-HRP Jackson Immuno Research 111-035-144 1:2000 – Alexa Fluor Donkey anti Rabbit 488 ThermoFisher Scientific A21206 – 1:500 Alexa Fluor Donkey anti Goat 555 ThermoFisher Scientific A21432 – 1:500 Alexa Fluor Donkey anti Mouse 546 ThermoFisher Scientific A10036 – 1:500 Open in a separate window List of antibodies used in this study and dilution 2.6 |.

    Techniques: Western Blot, Control